Int J Pharm Investig. Format), Citation-(BibTeX 2008;26(11):12618. WebTo address this perception, in the current study, FITC-dextran of different MWs (10, 70 and 150 kDa) was used to model the transdermal delivery of low MW biotherapeutics and mAbs with MWs of approximately 150 kDa. Weissella: An Emerging Bacterium with Promising Health Benefits. Huggins, Maurice L. (1942). Molecular weight (Mv) and hydrodynamic properties can be calculated from there. Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. Macromolecules, 31, 7712-7716. 2003;89(2):31120. Monolayers and Thin Films of Dextran Hydrophobically Modified. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). Pharm Res. 1994, 95-0355. Our portfolio includes for example derivatives of dextran, polysucrose, inulin and more. In the case of the work performed in references [34, 35, 36] they studied using the number average molecular weight, which rearranges the value of a and forces it to enter into unbranched or linear molecules, on the other hand, if molecular weights are used the value would be less than 0.5. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. Epub 2012 May 28. Here we have the hydrodynamic radius estimate for the 3kDa dextran and 1.5 kDa dextran by the Zetasizer calculator, thanks to Alan. Alan F Rawle, t 2016;47:15964. This study demonstrated that hydrodynamic volume and radius of gyration of a dextran sample with M w < 20 kDa and its linear counterpart with equal M w are almost ; Ortega, A.; Garcia de la Torre, J.; Harding, S.E. Ortega, A.; Garca de la Torre, J. Chem. Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. Smith, David R.; Raymonda, John W. (1972). The Solomon-Ciuta single-point method is used for calculating dextran intrinsic viscosity and the error relative is calculated, and %RE respect to Huggins method is >4.18% for 2000kDa, and >2.06% for 8.8kDa (Table 2). Viscotek GmbH, Europe. 2019;9(3-S):9726. Data File Dextran. Soc., 21, 1, 78-86. Synthesis of water-soluble and water-insoluble amphiphilic derivatives of dextran in organic medium. 2018 Aug 9;23(8):1984. doi: 10.3390/molecules23081984. All authors have consented to the submission of this manuscript for publication. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. Levin G, Gershonowitz A, Sacks H, Stern M, Sherman A, Rudaev S, Zivin I, Phillip M. Transdermal delivery of human growth hormone through RF-microchannels. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. Harding, Stephen E. (2005). The explanation for the anomalous values of a and kM-H is because the mixture solute-solvent is highly compatible due a great compaction of biopolymer, from undisturbed state in the absence of interactions, where free energy of mixing less than zero, and emphasize the nature non-linear or hyperbranched of dextran for Mw high to 200kDa. This situation is mainly the result of using dextans, solvent, additives, etc. 24h after microporation, the pore sizes reduced from 64.8817.20m (0h) to 48.854.27m (24h), and the pore depths reduced from 83.9120.56m (0h) to 63.9527.61m (24h). Format), Citation-(EndNote Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. Food Funct. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. CAS Progress in Polymer Science, 30, 38-70. Porcine wound models for skin substitution and burn treatment. ; Weisz, J. Carbohydrate Polymers, 74, 372-378. Solution properties of branched dextran. Determination of the intrinsic viscosity has been the most used measurement in the last 60 years. The skins were from the abdomen of a 39-year-old caucasian woman (BMI: 24). Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation. Theory, Estimation, Experiment, and Data. 2021 Aug;13(4):915-925. doi: 10.1007/s12602-021-09751-1. Lucifer yellow (LY) and FITC-labelled dextran stock solutions were prepared in Ringers-HEPES buffer (150 mM NaCl, 3.4 mM CaCl 2, 1.2 mM MgCl 2, 5.2 mM KCl, 0.5 mM NaHCO 3, 2.8 mM glucose, and 10 mM HEPES) and frozen at 20C. Strategies to improve the potential of transdermal devices by enhancing the skin permeation of therapeutic entities. Formul. Dextrans can function efficiently as anterograde or retrograde tracers, Pharmacol Pharm. Drug Deliv. The above data are not useful because it can not be compared with those obtained in this work. Biomacromolecules 8, 2464-2475. Pribush, A.; Zilberman-Kravits, D.; Meyerstein, N. (2007). Accueil; Solution; Tarif; PRO; Mon compte; France; Accueil; Solution; Tarif; PRO; Mon compte If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. Studies carried out at different ionic strength you can see them in the references [64, 65] and works with different solvents in the references [66, 67]. J. Phys. In this work, the human cadaver skin was treated to confirm the skin regeneration after the application of the microporation device. Pure Appl. WebHydrodyamic Radius, R H: In addition to the analytic size of a linear (C=1) chain, the end to end distance, R eted or R 0, and the structural size, the radius or gyration, R g, dynamic measurements yield a size called the hydrodynamic radius, R H. Consider a rod particle of length L=10R where R is the radius. A simple and rapid method for evaluation of MarkHouwinkSaturada constants of linear random coil polysaccharides using molecular weight and intrinsic viscosity determined by high performance size exclusin chromatography: application to guar galactomannan. Walkenhorst, S.; Olivier, R. (2002). FOIA Pig skins (Micropig Franz cell membrane) were supplied by Medi Kinetics Co., Ltd. (Pyeongtaek, Korea). Clipboard, Search History, and several other advanced features are temporarily unavailable. The human and pig skin permeability of FITC-dextran (M.W. government site. Macromolecules. J. Braz. It is worth noting that the size of macromolecules is much bigger than that of solvent (water) molecules [50]. The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. J Cosmet Laser Ther. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. Journal of Applied Polymer Science, 72, 871-876. 4K and M.W. Molecular Weight Distributions of Water-Soluble Polymers by Flow Field-Flow Fractionation. These vibrations heat the skin tissue, triggering water vapor and cell ablation, forming microchannels from the SC through to the outer dermis. Markiertes Dextran wird hufig per Mikroinjektion in die Zellen eingebracht. As intrinsic viscosity of dextrans decreases with an increase in solution temperature; chain flexibility enhances with an increase in temperature (see Table 4) showing that the intrinsic viscosity of dextran (with different molecular weights Mw from 8.8 to 200kDa) decreases linearly with an temperature increase in solution, but change to high molecular weight to hyperbranched macromolecule. (1969). (1954). Indo AM J Pharm. European Journal of Pharmaceutics and Biopharmaceutics, 68, 319-329. ; Wood, P.J. The skin was treated with the RF-based microporation device, inducing micropore ablation through alternating electrical current. Viet, D.; Beck-Candanedo, S.; Gray D. G. (2008). The numerical value of a indicates that dextrans acquire the shape of a rigid sphere in aqueous solution; and kM-H demonstrates that under water the value decreases with temperature [71, 72]. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. For 40 kDa molecules, the hydrodynamic diameter d h ~8 nm is much smaller than the collagen mesh size [33,41]. Unperturbed dimensions and the theta temperature of dextran in ethylene glycol solutions. For a limited temperature range, the viscosity of a polymer solution varies generally following a relation similar to that of usual liquids (rrhenius plot), where the polymer concentration is not very high and that temperature remains far enough from glass transition [38], and for system PEAorganic solvents see reference [63]. The solution viscosity () is related to the solvent viscosity (o), resulting in the relative viscosity. a Microscopy images of micropore and b quantified data using ImageJ after 0 (immediately), 4, 8, and 24h according to the number of times microproration was done (n=150, *: p<0.05). TEWL values before and immediately after microporation on human cadaver skin according to the number of times microproration was done (n=3). Carbohydrate Polymers 88 373-381. The analysis of errors using the Solomon-Ciuta method is compared with HM [59]. The value of kM-H in this work decreases with increasing temperature in contrast to published by reference [34] where kM-H increases. Dulbeccos Phosphate-Buffered Saline (DPBS) was purchased from Welgene (Gyeongsan, Korea). Chem. Universal size-independent quantities for the conformational characterization of rigid and flexible macromolecules. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Thermo Fisher Scientific. Probiotics Antimicrob Proteins. Sung-Wook Choi. Arond L.H. Unperturbed dimensions and theta temperature of dextran in aqueous solutions. Webdextran hydrodynamic radius. Arch Dermatol Res. The cumulative permeation of FITC-dextran 10 K was 4.82 0.32 M, in the human skin and 5.92 0.46 M in the pig skin. To improve drug permeability, micropore ablation using RF is performed by passing an alternating electrical current through the skin at a frequency higher than 100kHz. Amersham Biosciences, AA Edition, www.amershambiosciences.com. Biological skin substitutes for wound cover and closure. Bahary, W.S. J. Braz. Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. An official website of the United States government. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. The flattening of the curves at higher molecular weight is in agreement with an increased branching density. Unable to load your collection due to an error, Unable to load your delegates due to an error. Teixeira CG, Fusieger A, Milio GL, Martins E, Drider D, Nero LA, de Carvalho AF. Macromolecules, 33, 5730-5739. Eng. Hydrodynamic radii ( RH) were determined by dynamic light scattering. After 8h, the micropore became more distorted from the previous circle, suggesting that the micropores were closing. and transmitted securely. Masuelli, Martin Alberto. Surface morphology study of dextran by scanning electron microscopy showed the porous web like structure. The Hydrodynamic Radii of Macromolecules and Their Effect on Red Blood Cell Aggregation. Harding, Stephen E. (1997). Ed., 9(2), 85-90. These constants can be determined experimentally by measuring the intrinsic viscosity of several polymer samples for which the molecular weight has been determined by an independent method (i.e. A single frequency laser is directed to the sample contained in a cuvette. The lower molecular weight of FITC-dextran permeated more on both human and pig skin. Catiker, E.; Guner, A. At the same temperature, the hydrodynamic radius and the intrinsic viscosity are higher for different molecular weights, while these properties decrease with an increase of temperature at the same molecular weight (Figure 1). 2009;301(8):60913. Nair SS. However, the results did not show significant regeneration of micropore compared to previous in vivo studies which reported significant regeneration [25]. Masuelli, Martin Alberto (2013). dextran hydrodynamic radius. A simple way of universalizing the measurements is shown in figure 4 where there is a curve at each temperature for every molecular weight. 56 The linkage that the enzyme dextranase breaks down is indicated by the black arrow. Table 5 shows the data of Mark-Houwink parameters determined in figure 4 from linear regressions with 2 values higher than 0.9797. Sachdeva V, Banga AK. Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 14662, Republic of Korea, LG electronics, 19 Yangjae-daero 11-gil, Seocho-gu, Seoul, 06772, Republic of Korea, You can also search for this author in Ann Surg Oncol. Equivalent Radii and Ratios of Radii from Solution Properties as Indicators of Macromolecular Conformation, Shape, and Flexibility. Granath, K. A. a-d Haematoxylin/eosin-stained histological sections of human cadaver skin sample and e quantified data using ImageJ for different time periods after microporation. what will you do to keep amazon safe answers; amarsi assisted living; peter clarke, emilia clarke; personality characteristics of kiran mazumdar shaw; karina mitchell age; mirela romanian orphan died; dsa presidential endorsement; dextran hydrodynamic radius. J Control Release. Progr. In addition, we evaluated the regeneration of SC after microporation. Carbohydrate Polymers, 79, 380-390. volume24, Articlenumber:22 (2020) Comparison of single-point and dilution procedures. Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1), Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining. ; Dutt, N.V.K. Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. Synthesis of dextran by dextransucrase treated, sucrose-supplemented skimmed milk revealed the promising potential of dextran as a food additive. Normally, the skin loses very little water loss whereas damaged skin loses more [22]. This method utilizes promising strategies that have made a significant impact in transdermal delivery [6], they include: iontophoresis, microneedles, sonophoresis, radiofrequency (RF), and chemical enhancers [7,8,9,10,11]. The molecular weight and Simha number do not change in this temperature range (Mw from 8.8-200kDa), P slightly change, showing modifications in the hydrodynamic properties of the biopolymer in aqueous solution as [] and RH. WebTry out PMC Labs and tell us what you think. Bethesda, MD 20894, Web Policies The Intrinsic Viscosity of Biological Macromolecules. 2013;43(4):287303. The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. This device can generate microchannels from the SC through to the outer dermis and allow transdermal delivery of active agents. Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. ; Veldhuis, T.F.J. Molecular weight (M v) and hydrodynamic properties can be calculated from there. Journal Argentine Chemical Society 100 (2013) 25-34. Article Seven dextrans, provided by Sigma, with molecular weight of 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa. Drug Deliv. Carbohydrate Polymers 99, 199-207. 2014 Sep;5(9):2324-30. doi: 10.1039/c4fo00319e. Biotechnology Progress, 19, 544-548. Recent Pat. Sci. The formula is verified for different systems of polymer-solvent and the values are in accord with those obtained by extrapolation. Webprotein hydrodynamic radius was observed with increasing protein concentration (in the range 0.25-2 mM) in both the absence and presence of 8 M urea. If there are particles in the sample, the incident laser light gets scattered in all directions. Viscosity of in water solution polysaccharides depends on intrinsic characteristics of the biopolymer (such as molecular weight, volume, size, shape, surface charge, deformation facility, esterification degree, and galacturonic content) and on ambient factors (such as pH, temperature, ionic strength, solvent, etc.). Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Differences were considered statistically significant at p <0.05. 4K, b M.W. ; Garca de , J. Kirkland, J. J.; Dilks, C. H.; Rementer, S. W. (1992). A novel high dextran yielding Weissella cibaria JAG8 for cereal food application. Journal of Chemical Education, 49(8), 577-579. European Polymer Journal, 42, 1086-1092. 2012;3(1):208. The [] is connected to the dimensions of the biopolymer molecule isolated in a certain solvent. Radiofrequency is a well-known medical technique with expanded application in electrosurgery for laparoscopic medical procedures and in dermatology, for sebaceous glands ablation in acne treatment [12, 13], and treatment of photoaging skin symptoms, including wrinkles and hyperpigmentation [14]. Thus, it is accepted that, for every flexible-chain polymer in a (ideal) solvent, there is a universal value of 0 = 2.1-2.5x1023mol-1 [52]. The enhanced permeability through micropores was confirmed by Franz diffusion cell. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect. The hydrodynamic radius R h, defined as the radius of a hard sphere that diffuses at the same rate as the solute, takes these effects into account. PubMedGoogle Scholar. Transdermal drug delivery system for non-steroidal anti inflammatory drugs: a review. Use of the single point method is correct because it is based in the Huggins method (HM); owing the HM requiring many viscosity and density measurements (at least four) for each Mw, but Solomon Ciuta method with a single measure is sufficient. We will also conduct further studies on the regeneration of human cadaver skin and evaluate the possibilities of clinical trials. The biological activity of cationic liposomes in drug delivery and toxicity test in animal models. Google Scholar. Static and Dynamic Light-Scattering Solution Behavior of Pullulan and Dextran in Comparison. TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. In this study, we investigated the effectiveness of RF microporation technology in vitro, by evaluating the permeation of fluorescent-conjugated dextran (FITC-dextran) of various molecular weights through the human and pig skin after microporation, using a transdermal diffusion cell system. Im Tiefkhlgert (-5 bis -30C) lagern und vor Licht schtzen. Transdermal delivery is of great importance for the effective delivery of bioactive or therapeutic agents into a body. Journal Non-Newtonian Fluid Mech., 97, 295-301. However, the currently available transdermal systems are limited by low drug permeability across the skin due to the lipophilic barrier function of the stratum corneum (SC), the outermost skin layer of the skin [3,4,5]; the methods favor low molecular mass, and lipophilic molecules, making it difficult to exploit the transdermal route for large and water-soluble molecules such as proteins and peptides. Would you like email updates of new search results? The intrinsic viscosity calculation requires several concentrations, and because of this inconvenient will not use the Huggins method in this paper. Analyzing the values of the hydrodynamic properties of dextran in aqueous solution, it can be seen in Table 7, that all vary with the temperature. Webdextran hydrodynamic radius. Advances in Physical Chemistry, Vol. Just in case anyone wants to use a different molecular weight to estimate the hydrodynamic size: the calculator is integrated into the classic Zeta In this sense, the largest deviations were obtained for the highest molecular weight (580-2000kDa with values from 0.8467 to 0.7346). The value of P increases from 1.0272 to 1.2694 (spherical shape with tendency to spheroid for high Mw). Guner, A.; Kibarer, G. (2001). 2013, Article ID 360239, pp. The basic setup of a DLS instrument is shown in Figure 1. The Dextrans intrinsic viscosity is determined as a function of the molecular weight in water and NaOH 0.5 M see reference [1]. California Privacy Statement, WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. The fast increasing of these polyglucosans for medical, industrial and research purposes motivated a survey of the types obtainable. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). 10% neutral buffered formalin was purchased from Hisko (Gunpo, Korea). One day before the experiment, the skin was stabilized in the skin culture medium. Dextran Triple Detector Application Note 1, Viscotek GmbH, USA. These structural differences can significantly affect FITC-dextran delivery due to even after microporation [30]. The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. Analytical Chemistry. De Belder, A. N. (2001). Search Progress in Measurement, Interpretation and Application to Structure in Dilute Solution. We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Pavlov, G.M. European Polymer Journal, 36, 2143-2146. Figure2 shows TEWL values measured before and immediately following the treatment of human cadaver skin with the microporation device. Determination of constants, kM-H and a, from the intrinsic viscosity data, requires a series of monodisperse polymer samples with known molecular weight or a series of polydisperse polymer samples with known viscosity average molecular weights, Mv. This low polydispersity realizes that the Mark-Houwink equation is forced at a given Mw regardless of the hyperbranched nature of the dextran molecule. Transdermal drug delivery: overcoming the skins barrier function. Advances in Carbohydrate Analysis, 1, 63-144. II. Journal of Colloid Science, 31, 308-328. Webof overall macromolecular geometry and hydrodynamic draining. Privacy WebR H Hydrodynamic radius [m] The basic DLS setup. It is a measure of the width of molecular weight distributions (MWD) as well as probability of molecular weight distribution curve (GPC). Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3. A few percent of R (13) glucosidic linkages provides side chains which appear to be short. The human and pig skin samples were placed in the receiver chamber of the Transdermal diffusion cell system with the SC facing up and the donor chamber was fixed in place. Dextrans in Aqueous Solution. To overcome these limitations, microporation devices that act as therapeutic vehicles for delivering drug molecules across the skin barriers have been developed. These results are in agreement with a previous study which suggested that the microporation using radiofrequency causes disruption of the SC, enabling the percutaneous permeation of the FITC-dextran [21]. Table 1 shows some values of dextrans Mark-Houwink parameters, from literature consulted, where all data are based on the logarithmic plot of intrinsic viscosity [] and molecular weight Mw. Polysaccharides-based nanoparticles as drug delivery systems. WebFITC Products. The range of temperatures used was 25-50 (0.1) C and it was regulated by thermostatic bath Haake C. The viscosity measurements required Hagenbach-Couette correction time (tg 37.31s). Dextrans and their derivatives find an interest in clinical applications, as well as excipients in tablets in the pharmaceutical industry [5]. The proportionality between []0 and M0.5 is obtained as a limiting law for the no draining regime. Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! WebDextran 40 Pharmaceutical Quality. Please enable it to take advantage of the complete set of features! For a sphere and a random coil 0 the values are 9.23x1023 mol-1 and 2.60x 1023mol-1, respectively. In summary, we demonstrated effective enhancement of skin permeability and delivery of macromolecules lower than 20KM.W., and regeneration of human cadaver skin after microporation with an RF-based microporation device. 1905/05) for their financial support. This site needs JavaScript to work properly. Pamis, R.; Hernndez Cifre, J. G.; Lpez Martnez, M.C. Pharmaceut Sci Tech. The skin culture medium was purchased from Biopredic International (Saint-Grgoire, France). This is because the macromolecule hydrodynamic radius changes the solution type and temperature via change in their chain exibility. Saudi Pharm J. 2009;6(4):34354. Qi P, Caoa M, Songa L, Chena C, Liua M, Li N, Wua D, Penga J, Hub G, Zhao J. The values of 0 decreases from 2.5429 to 2.3326 x 1023mol-1 demonstrating a relative flexibility of particles. 2000;3(9):31826. 2006;3(3):37385. Drug Discov Today Technol. Ubbelohde, Leo. For Research Use Only. Determination of polymer structure by Gel Permeation Chromatography. WebThe study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. As a consequence of the diversity in their origin, the set of classical universal size independent quantities suffers from shortcomings. DLS tests were conducted at detection angle of 90 (standard) and 173 (backscatter) to measure protein hydrodynamic radius, while protein charge was examined in phase analysis light scattering (PALS) mode at detection angle of 15 (forward). There is a formula that used to calculate the radius (in Angstrom) from the molar mass (in g/mol) of dextran, a=0.33*(MM^0.46) where, a and MM are 2003;10:33847. Microneedles and their applications. The microporation device based on radiofrequency can be used to enhance delivery efficiency by removing the epidermis layer.
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